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A Supreme Court judge in New York will not prevent a COVID-19 vaccine mandate from taking effect for New York City's cops, dealing a blow to the city's largest police union, the Police Benevolent Association (PBA).

The ruling from Judge Lizette Colon, reported by the Associated Press, came after she heard arguments from the PBA, which filed a request for a temporary restraining order in hopes the mandate for the city's municipal workforce would be dismissed.

NYC WORKERS PROTEST VACCINE MANDATE: 'WE WILL NOT COMPLY!'

A New York City police officer.

A New York City police officer.

In addition to Colon's ruling on the mandate, the judge, according to the AP, ordered city officials to "appear in court Nov. 12 to defend the requirement against a union lawsuit seeking to have it declared illegal."

Municipal workers, including police officers, firefighters, garbage disposal workers and other city employees, will now be required to show proof that they have received at least their first dose of the COVID-19 vaccine by 5 p.m. ET Friday. Those who fail to comply with the mandate, pushed by Mayor Bill de Blasio and Police Commissioner Dermot Shea, will be put on unpaid leave beginning Nov. 1.

Mayor of New York City Bill de Blasio speaks during the opening of a vaccination center for Broadway workers in Times Square April 12, 2021 in New York City. (Photo by Noam Galai/Getty Images)

Mayor of New York City Bill de Blasio speaks during the opening of a vaccination center for Broadway workers in Times Square April 12, 2021 in New York City. (Photo by Noam Galai/Getty Images)

"Today’s ruling sets the city up for a real crisis," said PBA President Patrick J. Lynch. "The haphazard rollout of this mandate has created chaos in the NYPD. City Hall has given no reason that a vaccine mandate with a weekly testing option is no longer enough to protect police officers and the public, especially while the number of COVID-19 cases continues to fall."

Lynch said "the NYPD’s leadership spins its wheels and offers no guidance" as officers are "being told to make a possibly life-changing decision in a matter of days to meet a completely arbitrary deadline."

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Police Benevolent Association of the City of New York President Patrick Lynch. (Photo by Chip Somodevilla/Getty Images)

Police Benevolent Association of the City of New York President Patrick Lynch. (Photo by Chip Somodevilla/Getty Images)

"This not only violates police officers’ rights, it will inevitably result in fewer cops available to protect our city," Lynch concluded.