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Hundreds of churches split from a regional body of the United Methodist Church (UMC) over the weekend as the second-largest Protestant denomination in the U.S. continues to hemorrhage congregations.

Delegates at a special session of the UMC's North Carolina Conference voted 957-165 on Saturday to approve the departure of 249 conservative congregations in the regional body that wanted to be disaffiliated from the mainline denomination effective Dec. 31.

The number of churches leaving is roughly 32% of the conference's member churches, and they are taking with them about 22% of its overall membership, according to a statement.

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Protesters at UMC General Conference

Protesters chant during the United Methodist Church's special session of the General Conference in St. Louis, Missouri, on Feb. 26, 2019. (AP Photo/Sid Hastings)

The churches are the latest to depart from the UMC amid an ongoing schism over issues of sexuality. Fifty-eight churches in the Louisiana Conference disaffiliated during a special session earlier this month, and 70 congregations disaffiliated from the North Georgia Conference in June.

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Prominent Methodist churches in Texas, Arkansas and Louisiana have also expressed their intention to leave.

The UMC has faced a growing divide in recent years as conservative Methodist congregations push back against liberal ones they claim are being disobedient to the church.

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Rev. Cynthia Good at Calvary United Methodist Church

The Rev. Cynthia Good, pastor at Calvary United Methodist Church in Arlington, Massachusetts, speaks to her church during Sunday services on Jan. 5, 2020. (Jessica Rinaldi/The Boston Globe via Getty Images)

At a special session of the General Conference in 2019, the UMC voted 438-384 to uphold the church’s ban on ordaining LGBTQ clergy and officiating at or hosting same-sex marriages.

The General Conference also adopted a disaffiliation agreement that year that offered a runway for churches to leave the denomination through the end of 2023 "for reasons of conscience" related to the practice of homosexuality or the ordination or marriage of "self-avowed practicing homosexuals," which is forbidden in the church's Book of Discipline.

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Several conservative members of the Methodist clergy told Fox News Digital in June that far from settling the issue, however, the 2019 vote has been disregarded by many liberal leaders within the UMC who have opted to stay in the denomination while commissioning openly gay clergy and officiating same-sex weddings anyway.

LGBTQ flag Methodist church

A LGBTQ+ flag flies over Union United Methodist Church in Boston on Jan. 5, 2020. (Jessica Rinaldi/The Boston Globe via Getty Images)

Thomas Lambrecht, the vice president of the denomination's traditionalist Good News caucus, said at the time that the centrists and progressives in the UMC who chose to defy the church instead of leaving have rendered it "essentially ungovernable."

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Many of the congregations that depart the UMC have gone on to join the conservative Global Methodist Church, which launched on May 1 as a conservative alternative for Methodist churches around the world that want to break off from the increasingly liberal UMC.