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Joseph diGenova has resigned from his Justice Department role investigating the alleged "Grand Conspiracy" surrounding the Trump-Russia investigations, according to two sources familiar with the matter. 

Fox News has learned diGenova had lost the confidence of both the Justice Department and the White House. According to sources familiar with the matter, he had been effectively sidelined well before his departure.

His resignation comes after months in which his role had diminished internally, ending his involvement in the investigation amid questions about his standing within both the administration and DOJ leadership.

ABC News first reported his resignation. 

"It was an honor and a privilege to serve the president and the department," diGenova told CBS News. 

He declined to comment further on the circumstances surrounding his departure.

DiGenova’s wife, attorney Victoria Toensing, who also joined the federal prosecution team in South Florida, is expected to leave the Justice Department on Friday, according to CBS News.

Joseph diGenova, who has resigned from his role at the Department of Justice.

Joseph diGenova, who has resigned from his role at the Department of Justice. (Photo By Tom Williams/CQ-Roll Call, Inc via Getty Images)

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The so-called "Grand Conspiracy" investigation is based out of the U.S. Attorney’s Office in Fort Pierce, Florida. It examines whether Obama- and Biden-era officials were involved in a long-running effort to prevent Trump from returning to political office.

The probe has scrutinized officials involved in past investigations of Trump, including former CIA Director John Brennan and former FBI Director James Comey. DiGenova was also overseeing a separate criminal investigation into whether Brennan lied to Congress about the intelligence community’s assessment that Russia interfered in the 2016 election.

Rudy Giuliani is seen alongside Joseph diGenova during a news conference at the RNC.

Rudolph Giuliani, left, and Joseph diGenova, center, attorneys for President Donald Trump, conduct a news conference at the Republican National Committee on lawsuits regarding the outcome of the 2020 presidential election on Thursday, November 19, 2020 (Tom Williams/CQ-Roll Call/Getty Images)

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The investigation has issued dozens of subpoenas over the past year. Lawyers for some witnesses were recently notified that their clients would receive grand jury subpoenas, while investigators also sought voluntary interviews with former FBI agents involved in the Mar-a-Lago investigation, according to CBS News. Prosecutors have not filed charges in either case.

DiGenova was tapped to oversee the probe in April after career prosecutor Maria Medetis Long was removed from the case. He had criticized delays in the investigation and said indictments could have been brought within 30 days.

His departure, along with Toensing’s expected exit, leaves the future of the investigation uncertain.

Joe Digenova FOX

Joe Digenova on Fox News in 2019.  (Fox News)

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The longtime Washington attorney previously served as U.S. attorney for the District of Columbia from 1983 to 1988. He later represented Trump’s campaign during its unsuccessful effort to challenge the 2020 election results.

This is a developing story. Check back later for updates.