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Former NFL star Demaryius Thomas, who died last December at age 33, had CTE, his family said Tuesday.

Boston University CTE Center researchers diagnosed the four-time Pro Bowl wide receiver with stage 2 chronic traumatic encephalopathy following a brain study through the Concussion Legacy Foundation. Thomas’ family released the findings of the study.

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Demaryius Thomas runs a route

In this Sept. 16, 2018, file photo, Denver Broncos wide receiver Demaryius Thomas (88) runs against the Oakland Raiders during the second half of an NFL football game, in Denver. Former NFL star Demaryius Thomas, who died last December at age 33, had CTE, his family said Tuesday, July 5, 2022.  (AP Photo/Jack Dempsey, File)

"Once I became aware of CTE and began to familiarize myself with the symptoms, I noticed that Demaryius was isolating himself and I saw other changes in him," Katina Smith, Demaryius’ mother, said in a statement. "He was just so young, and it was horrible to see him struggle. His father and I hope all families learn the risks of playing football. We don’t want other parents to have to lose their children like we did."

Stage 2 CTE is associated with "progressive behavior, cognitive and mood abnormalities." Family members say Thomas developed depression, anxiety, panic attacks, and trouble with his memory in the years before his death. Stage 4 is the most severe stage of CTE and is usually associated with dementia.

"Like so many that have gone before, we found stage 2 CTE in the brain of Demaryius Thomas. The question I keep asking myself is ‘When will enough be enough?’ When will athletes, parents and the public at large stop ignoring the risks of American football and insist that the game be changed to reduce subconcussive hits?" said Dr. Ann McKee, chief of neuropathology for the VA Boston Healthcare System and director of the BU CTE Center and VA-BU-CLF/UNITE Brain Bank.

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Demaryius Thomas tribute on the field

A detailed view of a ball on the #88 tribute logo to the late former Denver Broncos player Demaryius Thomas after the game against the Detroit Lions at Empower Field At Mile High on December 12, 2021 in Denver, Colorado.  (Photo by Justin Edmonds/Getty Images)

CLF co-founder and CEO Dr. Chris Nowinski arranged the study through Thomas’ family.

"The football community would have no idea why so many former players struggle with neurological disorders after their career without the families who say yes to brain donation, so I want to thank Bobby Thomas and Katina Smith — and all families — for their trust in Dr. McKee and this team," said Nowinski, a former football player at Harvard. "I hope this is a wake-up call to high profile current and former NFL players that CTE is rampant among them, and they need to get involved in creating real solutions. CTE should be their number one off-the-field issue."

Thomas is one of more than 300 former NFL players who have been diagnosed with CTE by McKee and the BU CTE Center research team.

Thomas’ cause of death has not yet been identified. In 2021, Thomas began experiencing seizures. McKee told ABC News she believes the seizures were due to severe traumatic injuries off the football field, including a car wreck and a fall on stone stairs.

"CTE itself does not cause death. You don’t die from CTE. What CTE does is it changes your behavior and your personality," McKee said.

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Demaryius Thomas catches football

Denver Broncos wide receiver Demaryius Thomas (88) catches a pass before an NFL football game against the Seattle Seahawks in Denver, Sept. 9, 2018. Former NFL star Demaryius Thomas, who died last December at age 33, had CTE, his family said Tuesday, July 5, 2022.  (AP Photo/Jack Dempsey, File)

Thomas played 10 seasons in the NFL for the Denver Broncos, New York Jets, and Houston Texans. He won a Super Bowl with the Broncos and Peyton Manning following the 2015 season.

Thomas had 724 catches for 9,763 yards and 63 touchdowns.