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India summoned the Canadian Deputy High Commissioner on Monday and expressed "deep concern and strong protest" after separatist slogans in support of a Sikh homeland were raised at an event addressed by Prime Minister Justin Trudeau.

Bilateral diplomatic relations soured last year after Trudeau said Canada was "actively pursuing credible allegations" that Indian agents were potentially linked to the June 2023 murder of a Canadian citizen.

Hardeep Singh Nijjar, 45, was shot dead outside a Sikh temple on June 18 in Surrey, a Vancouver suburb with a large Sikh population. Nijjar supported a Sikh homeland in the form of an independent Khalistani state and was designated by India as a "terrorist" in July 2020.

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New Delhi has denied any formal government role in Nijjar's murder.

A group of protesters hold yellow flags with the word Khalistan, as well as a banner with the picture of Sikh separatist leader Hardeep Singh

A group of protesters hold yellow flags with the word "Khalistan," as well as a banner with the picture of Sikh separatist leader Hardeep Singh, during a protest outside India's consulate, a week after Canada's Prime Minister Justin Trudeau raised the prospect of New Delhi's involvement in the murder of Sikh separatist leader Hardeep Singh Nijjar in British Columbia, in Toronto, Ontario, Canada, on Sept\. 25, 2023.  (Reuters/Carlos Osorio/File Photo)

India's foreign affairs ministry said on Monday it had conveyed "deep concern and strong protest" at such actions "being allowed to continue unchecked at the event".

Slogans supporting the rise of a separatist state were raised at an event in Toronto, according to ANI news agency, in which Reuters has a minority stake.

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"We will always be there to protect your rights and your freedoms, and we will always defend your community against hatred and discrimination," ANI reported Trudeau as saying.

Canada has the highest population of Sikhs outside their home state of Punjab in India, and the country has been the scene of many demonstrations that have irked India.

The Canadian foreign ministry did not immediately respond to a request for comment.